; The top 3 importers of Spectramax are India with 365. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. Multimode Readers Comparison Chart. The following document is intended to demonstrate setup of this instrument and provide representativeUsed together with the SpectraMax iD3 reader, the EarlyTox Cell Viability Assay Kits enable direct measurement of live and dead or apoptotic cells with a simple workflow and the increased throughput offered by a microplate format. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Read More. 000). Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. SpectraMax ABS Plus SpectraTest ABS2 v1. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. New drivers take 6 days to write, existing drivers take 2 days to convert to Windows 7/10 compatibility. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. . Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The excitation and the emission wavelengths on. 6. acquired on a SpectraMax M5 (Molecular Devices). The drawing shows a standard microplate of 15 mm height covered with a lid. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 0 mL). The ability to measure inhibition of phosphorylation by Erlotinib was tested by incubating cells with increasing concentrations of Erlotinib, then stimulating cells with 0. Open the Softmax Pro software and select the FP option. ORDER. SpectraMax M5 and the Analyst GT. SpectraMax Injector Cartridge with SmartInject™ Technology. 9000-0756. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. The following document is intended to demonstrate setup of this instrument. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader. Sensitive fluorescent quantitation of DNA with the Quant-iT. Read fluorescence polarization at room temperature (RT, 19–25°C). ItSpectraMax® M5多功能读板机为使用者提供了除化学发光以外的其他检测方法，例如：光吸收，荧光强度，荧光偏振和时间分辨荧光。 SpectraMax® L和SpectraMax® M5酶标仪均用SoftMax® Pro软件进行操控和设置，该软件含有CellTiter-Glo检测模板和其他120多个分析模板，这些. Time-Resolved Fluorescence (TRF) Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing. . For example, microplates containing different samples can be read using theThe interval between reads was set at 15 s or 60 s, respectively. These robust readers have been placed in labs from Antarctica to the. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. SpectraMax M5, Analyst HT or Analyst GT. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Cytokines in medium released. Five preferred modes for diverse bioresearch applications. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. FlexStation® 3 Multi-Mode Microplate Reader. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. 4. 0 out of 5 $ 15,999. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. The guide also covers the software features, the specifications, and the accessories of the systems. Description 1. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Step 4. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. the IMAP Platform and smoothly transition into high-throughput screening. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. 6: bioNova científica, s. The ratioof the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%)As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. SpectraMax Glo and DuoLuc. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. These all-inclusive assay kits are ready to use in assay validation or screening. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. . SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. PAINS analysisThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The M5 has two PMTs. 4. 0 out of 5 $ 5,999. Fluorescent Polarization: Solid black plates, as above. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. Table of Contents. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. SpectraMax Quant dsDNA Assay Kits. For example, microplates containing different samples can be read using the312 gain calibration failed. Figure 1. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. EUROPE. e. 3. 1). Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. The SpectraMax M5 is a microplate reader that can be used for various applications,. View the Molecular Devices websiteSpectraMax® M5 multimode microplate reader from Molecular Devices to measure both reporters in a completely homogeneous manner, in the same well of a 96-well plate, without cell lysis. The REF button has no valid meaning for. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. The dynamic range of the assay was approximately 5 orders of magnitude. Table of Contents. type Clear. 1) EarlyTox Glutathione Assay (6. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. . Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. 4. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. 1. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. • Tel. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . The chamber contains springs that position the cuvette in the proper alignment for a read. 95. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. NORTH AMERICA. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. 7" color, capacitive touch screen. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Step 3. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. $4,499. Description 1. Step 4: Incubate at room temperature for 1 hour, protected from light. 1. SpectraMax Microplate Readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. . The absorbance of MPO was calculated using the SpectraMax M5 (Molecular Devices Corporation, San Jose, CA, USA) at 460 nm. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. 2. 00 USD. . The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Wash Buffer Concentrate was diluted 10-fold. Add to Cart. Sale. The Spectr. The KeySpan USA-19HS is often required with new Windows 7. D 1. Set the excitation at 531 nm and emission at 595 nm. Also refer to this Multimode Reader Comparison Chart. 00 USD. Fluorescence Microplate Reader Comparison Chart. Lift up the lid over the chamber to insert or remove a cuvette. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The. 0 (IBM Corporation, Armonk, NY, USA). Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. com A. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. When the user applies a set temperature of 37°C, the top is heated to 37. 1) SpectraMax Glo Steady Luc Reporter Assay (6. The time course of the cleavage reaction was monitored by loss of FRET (excitation, 450 nm; emission, 655 nm) with 15 reads/well and 4–5-s read intervals on a SpectraMax M5 plate reader (Molecular Devices). 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. Choose from a three- (M3), four- (M4), orThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Cables. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. . Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. 2. edu) to be added as a trained user in iLab. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. 19. The SoftMax® Touch Software is installed in. . When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SoftMax Pro Software combines reader control and data analysis and provides sophisticated curve fitting and statistics for more in-depth bioassay analysis. 多功能酶标仪SpectraMax M5e. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionHere, we present FluoroBrite™ DMEM, a cell culture media designed specifically for live-cell imaging that, when excited at wavelengths commonly used for FITC/GFP (i. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 306 plate air cal fail,low light. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. Molecular Devices also offers a mass spectrometry-based solution for food safety testingMOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. . The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Absorbance measurements of ADMA were acquired using a Biotek Cytation 5-plate reader. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Molecular Devices SpectraMax 190 Microplate Absorbance Spectrophotometer. The . 00 USD. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Pick the best speed, sensitivity, and price for your needs. The plate reader. view more . . SoftMax® Pro Microplate Data Acquisition and Analysis Software serves as the user interface to the microplate reader. Setup recommendations for SpectraMax® M5e. For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic Gq-coupled receptor. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax® M5 Multi-Mode Microplate Reader is capable of measuring UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, and TRF. 190-1000 nm, tunable in 1 nm increments. Molecular Device SpectraMax M5 Plate Reader - Certified with Warranty. Access this by going to Help >. 5. Samples were analyzed in eight to sixteen wells per independent. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. spr SpectraMax iD3 SpectraTest ABS2 v1. . Customer. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeWe would like to show you a description here but the site won’t allow us. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. For example, microplates containing different samples can be read using the The interval between reads was set at 15 s or 60 s, respectively. Spectranet customer portalMice were fasted overnight and orally administered FITC-dextran (600 mg/kg). D 1 1. 4. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. . Plate chamber design of the SpectraMax category 1 microplate readers. Purchase Overlord with the required number of. 3: The cuvette chamber. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesand emission wavelengths for SpectraMax M5 fluorescence microplate readers are as follows: excitation at 490 nm and emission at 525 nm, with 515-nm emission cutoff filter (note that these are different from the wavelengths recommended for filter-based readers in the Quant-iT PicoGreen product insert). Add to Cart. SoftMax Pro software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The chamber. $2,264. The assay applies fluorescence polarization to quantify Fc-containing IgG. SpectraMax Spectrofluorometers. D 1. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5, Analyst HT or Analyst GT. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Here, we present FluoroBrite™ DMEM, a cell culture media designed specifically for live-cell imaging that, when excited at wavelengths commonly used for FITC/GFP (i. 313 reference gain check fail. e. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. 1. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. Wavelength Range. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The following document is. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). This application note describes how to. The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. SOFTmax PRO UserÕs Manual iii Contents Figures . $1,250. Table of Contents. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. Step 4: Incubate at room temperature for 1 hour, protected from light. There were 12 replicate wells per dilution in both 96-well and 384- well plates. 306 plate air cal. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. 3. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Italic and boldface type are used for emphasis. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. viiProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader What is the best way to use fluorescence (FL) mode with cuvettes? 1. 9000-0756. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Refer to “Installing Detection Cartridges” in. In addition, the SpectraMaxMolecular Devices. Description 1. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. com A. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Detection modalities are shown in Table 1-1. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. The following document is intended to demonstrate setup of this. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. Issue: The audit trail in a SoftMax Pro GxP Software data file records a user as logging out of the system when the Auto Log Off prompt. 5. . Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. 5. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. Proceed with the following steps to optimize the instrument: 1. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. 0 mm wide, and heights from 14 mm to 15 mm. SoftMax Pro. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Published Dec 16, 2020 | Updated Dec 16, 2020. Bookings are free, but you will need to use iLab to book. Choose from a three- (M3), four- (M4), or Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Choose from a three- (M3), four- (M4), orAll general chemicals and solvents were commercially available and used as received. Catalog Number: M5. Weight. 3: The cuvette chamber. Features. It does not work as expected. Detection of pro-inflammatory factors. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. These lab workhorses are used in drug discovery, research, bioassay validation, and. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Choose from a three- (M3), four- (M4), orSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. UV/Vis absorbance and fluorescence spectra were measured on SpectraMax® M5 Multi- Mode Microplate Reader. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. . 7. 7°C (gradient heating design). Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. 5. 005) Photometric PrecisionThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. 1step assay with SpectraMax microplate readers. Data Acquisition & Analysis Software. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. 4. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. If you do not have an iLab account you must first make one: SpectraMax Paradigm Fluo rescence Polarization (FP) Detection Cartridge is a single slot detection cartridge and occupies one (1) slot. 7°C and the bottom to 36. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. , contact Molecular. Refurbished. free shipping. The drawing shows a standard microplate of 15 mm height covered with a lid.